Creb1^tm4Gsc
Targeted Allele Detail

Summary

• Symbol: Creb1^tm4Gsc
• Name: cAMP responsive element binding protein 1; targeted mutation 4, Gunther Schutz
• MGI ID: MGI:2183908
• Synonyms: CREB^S142A
• Gene: Creb1 Location: Chr1:64571963-64643707 bp, + strand Genetic Position: Chr1, 32.74 cM, cytoband C1/C5
• Alliance: Creb1^tm4Gsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75993
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A serine to alanine substitution at amino acid 142 (S142A) was introduced to exon 8 and a floxed TK-neo cassette was inserted into the upstream intron via homologous recombination. Removal of the TK-neo cassette was achieved by transient expression of cre recombinase in correctly targeted ES cells. The presence of the S142A mutation was verified by sequencing of RT-PCR products from heterozygous animals. Immunohistochemistry of brain sections from homozygous mutant animals demonstrates the absence of phosphorylation at serine 142. The gene expression pattern of homozygous mutants is similar to that of wild-type animals. (J:75993)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Creb1 Mutation: 61 strains or lines available

References

• Original: J:75993 Gau D, et al., Phosphorylation of CREB Ser142 Regulates Light-Induced Phase Shifts of the Circadian Clock. Neuron. 2002 Apr 11;34(2):245-53
• All: 2 reference(s)