Klf4^tm1Efu
Targeted Allele Detail

Summary

• Symbol: Klf4^tm1Efu
• Name: Kruppel-like transcription factor 4 (gut); targeted mutation 1, Elaine Fuchs
• MGI ID: MGI:2183936
• Synonyms: Klf4-
• Gene: Klf4 Location: Chr4:55527143-55532466 bp, - strand Genetic Position: Chr4, 29.76 cM
• Alliance: Klf4^tm1Efu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56495
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.8 kb region of the gene encompassing part of exon 1, exon 2, and exon 3 was replaced with a PGK-neo cassette via homologous recombination. The targeted region encodes the translation initiation codon and the first of 3 zinc finger domains. Northern blot analysis and immunohistochemistry of skin sections from homozygous mutant animals verified the absence of gene expression. (J:56495)

Expression

In Mice Carrying this Mutation:	51 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klf4 Mutation: 26 strains or lines available

References

• Original: J:56495 Segre JA, et al., Klf4 is a transcription factor required for establishing the barrier function of the skin. Nat Genet. 1999 Aug;22(4):356-60
• All: 10 reference(s)