Tg(Fabpl-RAC1-T17N)1Jig
Transgene Detail

Summary

• Symbol: Tg(Fabpl-RAC1-T17N)1Jig
• Name: transgene insertion 1, Jeffrey I Gordon
• MGI ID: MGI:2383956
• Synonyms: Rac1Asn17
• Transgene: Tg(Fabpl-RAC1-T17N)1Jig Location: unknown
• Alliance: Tg(Fabpl-RAC1-T17N)1Jig page

Transgene origin

• Mouse Generated: Earliest citation of chimera generation: J:62539
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Fabpl-RAC1-T17N)1Jig expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RAC1 (5879)

  Rac1	(MGI:97845)

  expresses a dominant negative threonine to asparagine mutation at amino acid 17

• Tg(Fabpl-RAC1-T17N)1Jig expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Fabp1 (24360)

  Fabp1

  (MGI:95479)

• Mutation details: A human RAC1 gene carrying a dominant negative threonine to asparagine mutation at amino acid 17 was used for the transgene. Nucleotides -596 to +21 from rat Fabpl were used to drive expression of the transgene in intestinal epithelium. (J:62539)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Transgenic animals exhibited abnormal morphology of jejunal crypt-villus units, inhibition of epithelial differentiation, and slowing of epithelial migration.

References

• Original: J:62539 Stappenbeck TS, et al., Rac1 mutations produce aberrant epithelial differentiation in the developing and adult mouse small intestine. Development. 2000 Jun;127(12):2629-42
• All: 1 reference(s)