Grk5^tm1Rjl
Targeted Allele Detail

Summary

• Symbol: Grk5^tm1Rjl
• Name: G protein-coupled receptor kinase 5; targeted mutation 1, Robert J Lefkowitz
• MGI ID: MGI:2383968
• Synonyms: grk5^-, GRK5 KO, GRK5-KO
• Gene: Grk5 Location: Chr19:60878187-61084406 bp, + strand Genetic Position: Chr19, 56.52 cM
• Alliance: Grk5^tm1Rjl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59179
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 7 and a floxed TK-neo derived cassette was inserted downstream of exon 8 via homologous recombination. Exons 7 and 8 encode critical subelements I, II, and III of the protein kinase catalytical domain. The floxed region containing exons 7 and 8 and the TK-neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. Western blot analysis of brain membranes from homozygous mutant animals confirmed the absence of gene expression. (J:59179)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grk5 Mutation: 44 strains or lines available

References

• Original: J:59179 Gainetdinov RR, et al., Muscarinic supersensitivity and impaired receptor desensitization in G protein-coupled receptor kinase 5-deficient mice. Neuron. 1999 Dec;24(4):1029-36
• All: 22 reference(s)