Fgr^tm1Hev
Targeted Allele Detail

Summary

• Symbol: Fgr^tm1Hev
• Name: FGR proto-oncogene, Src family tyrosine kinase; targeted mutation 1, Harold E Varmus
• MGI ID: MGI:2384040
• Synonyms: fgr^-, fgr-deltaE2
• Gene: Fgr Location: Chr4:132701406-132729204 bp, + strand Genetic Position: Chr4, 66.11 cM
• Alliance: Fgr^tm1Hev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:16994
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 2 was disrupted by the insertion of a neomycin selection cassette. While Northern blot analysis of LPS activated macrophages showed the absence of normal and truncated transcripts, elongated transcript containing the neo transgene was detected in homozygous mutant mice. Normal protein was undetected by Western blot analysis, and though the absence of truncated carboxy terminal peptide was not directly confirmed, assays of bone marrow derived macrophages verified a lack of protein kinase activity. (J:16994, J:102347)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgr Mutation: 132 strains or lines available

References

• Original: J:16994 Lowell CA, et al., Functional overlap in the src gene family: inactivation of hck and fgr impairs natural immunity. Genes Dev. 1994 Feb 15;8(4):387-98
• All: 53 reference(s)