Flt3^tm1Irl
Targeted Allele Detail

Summary

• Symbol: Flt3^tm1Irl
• Name: FMS-like tyrosine kinase 3; targeted mutation 1, Ihor R Lemischka
• MGI ID: MGI:2384051
• Synonyms: Flk2^-
• Gene: Flt3 Location: Chr5:147267551-147337299 bp, - strand Genetic Position: Chr5, 86.88 cM, cytoband G3
• Alliance: Flt3^tm1Irl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:27238
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 3 was disrupted by the insertion of a neomycin selection cassette at the codon corresponding to residue 59 in the mature protein. An RNAse protection assay detected low levels of transcript in homozygous mutant mice. RT-PCR and sequence analyses of the mutant transcript showed that the targeted exon 3 had been excised, resulting in a frameshift mutation that introduces a stop codon after codon 51. (J:27238)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Flt3 Mutation: 90 strains or lines available

References

• Original: J:27238 Mackarehtschian K, et al., Targeted disruption of the flk2/flt3 gene leads to deficiencies in primitive hematopoietic progenitors. Immunity. 1995 Jul;3(1):147-61
• All: 43 reference(s)