Pafah1b1^tm1Awb
Targeted Allele Detail

Summary

• Symbol: Pafah1b1^tm1Awb
• Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; targeted mutation 1, Anthony Wynshaw-Boris
• MGI ID: MGI:2384054
• Synonyms: Lis1-, Lis-Neo, Pafah1b1^ex6neo-8, Pafah1b1-neo
• Gene: Pafah1b1 Location: Chr11:74564775-74615210 bp, - strand Genetic Position: Chr11, 45.76 cM, cytoband B3
• Alliance: Pafah1b1^tm1Awb page

Disruption of hippocampal layers in Lrp8^tm1Her/Lrp8^tm1Her, Pafah1b1^tm1Awb/Pafah1b1⁺, and Lrp8^tm1Her/Lrp8^tm1Her Pafah1b1^tm1Awb/Pafah1b1⁺ mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49531
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Part of exon 6 and all of exon 7 were replaced with a PGK-neo cassette in the opposite orientation via homologous recombination. The targeted mutation results in deletion of amino acids 164-214 and introduces multiple stop codons into exon 6. Homozygous mutant embryos were observed between E5.5 and E7.5 via Southern blot analysis. (J:49531)

Disease models

Expression

In Mice Carrying this Mutation:	4 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pafah1b1 Mutation: 160 strains or lines available

References

• Original: J:49531 Hirotsune S, et al., Graded reduction of Pafah1b1 (Lis1) activity results in neuronal migration defects and early embryonic lethality [see comments]. Nat Genet. 1998 Aug;19(4):333-9
• All: 37 reference(s)