Pafah1b1^tm2Awb
Targeted Allele Detail

Summary

• Symbol: Pafah1b1^tm2Awb
• Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1; targeted mutation 2, Anthony Wynshaw-Boris
• MGI ID: MGI:2384060
• Synonyms: Lis1-loxP, Pafah1b1-loxP, Pafah1b1^loxPex3-6
• Gene: Pafah1b1 Location: Chr11:74564775-74615210 bp, - strand Genetic Position: Chr11, 45.76 cM, cytoband B3
• Alliance: Pafah1b1^tm2Awb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49531
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A floxed PGK-neo cassette was inserted into intron 2 and a third loxP site was introduced into intron 6 via homologous recombination. This allele is hypomorphic, because of disruption of transcription or splicing by the insertion of a phosphoglycerate kinase neomycin cassette from the targeting vector into the second intron (J:82268). (J:49531)

Disease models

Expression

In Mice Carrying this Mutation:	6 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pafah1b1 Mutation: 160 strains or lines available

References

• Original: J:49531 Hirotsune S, et al., Graded reduction of Pafah1b1 (Lis1) activity results in neuronal migration defects and early embryonic lethality [see comments]. Nat Genet. 1998 Aug;19(4):333-9
• All: 24 reference(s)