Tfpi^tm1Gjb
Targeted Allele Detail

Summary

• Symbol: Tfpi^tm1Gjb
• Name: tissue factor pathway inhibitor; targeted mutation 1, George J Broze
• MGI ID: MGI:2384068
• Synonyms: TFPI^-, TFPI(desK1), TFPI(K1)^-
• Gene: Tfpi Location: Chr2:84263199-84307119 bp, - strand Genetic Position: Chr2, 49.41 cM
• Alliance: Tfpi^tm1Gjb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41945
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A region containing a portion of exon 4, encoding the first Kunitz domain, and intron 4 was replaced with a neomycin selection cassette. A truncated peptide, putatively translated from an aberrantly spliced transcript lacking sequence encoding the Kunitz domain, was detected in heterozygotes by Western blot analysis. Densitometric analysis of the blot showed the level of mutant protein in heterozygotes to be approximately 41% of the level of normal protein in wild-type mice. Functional activity was found to be reduced by approximately 46% in heterozygous mutant mice and 100% in homozygotes. (J:41945, J:51137)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tfpi Mutation: 47 strains or lines available

Notes

ES cells were either RW-4 (129X1/SvJ) or R1 (129S1/Sv x 129X1/SvJ). J:41945

References

• Original: J:41945 Huang ZF, et al., Tissue factor pathway inhibitor gene disruption produces intrauterine lethality in mice. Blood. 1997 Aug 1;90(3):944-51
• All: 15 reference(s)