Ccr10/Cntnap1^tm1Bhat
Targeted Allele Detail

Summary

• Symbol: Ccr10/Cntnap1^tm1Bhat
• Name: contactin associated protein-like 1; targeted mutation 1, Manzoor A Bhat
• MGI ID: MGI:2384080
• Synonyms: Caspr^-, Cntnap1^tm1Bhat
• Gene: Cntnap1 Location: Chr11:101065429-101081550 bp, + strand Genetic Position: Chr11, 64.33 cM, cytoband D
• Alliance: Ccr10/Cntnap1^tm1Bhat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69621
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A targeting vector disrupted two genes via homologous recombination. Regulatory sequences and the first 7 exons of the Cntnap1 gene were replaced with a neomycin resistance cassette, as were the first two coding exons of the Ccr10 gene. Loss of Cntnap1 gene expression in homozygous mutants was demonstrated by Western blot analysis of whole brain and sciatic nerve homogenates and by immunohistochemistry of sciatic nerves and CNS. Effects on Ccr10 gene expression were not examined though it was predicted to be a null allele due to the removal of the transcription start site for the gene. (J:69621)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cntnap1 Mutation: 56 strains or lines available

References

• Original: J:69621 Bhat MA, et al., Axon-glia interactions and the domain organization of myelinated axons requires neurexin iv/caspr/paranodin. Neuron. 2001 May;30(2):369-83
• All: 8 reference(s)