Fgg^tm1Jld
Targeted Allele Detail

Summary

• Symbol: Fgg^tm1Jld
• Name: fibrinogen gamma chain; targeted mutation 1, Jay L Degen
• MGI ID: MGI:2384081
• Synonyms: Fggammadelta5, gamma-delta5
• Gene: Fgg Location: Chr3:82915031-82922356 bp, + strand Genetic Position: Chr3, 36.94 cM
• Alliance: Fgg^tm1Jld page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36873
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A nonsense mutation was introduced at the last codon of exon 9 via site directed mutagenesis and subsequent homologous recombination, precluding the translation of 5 carboxy terminal residues (407 through 411). Due to its position at the 3' end of exon 9, the mutation does not affect residues involved in transglutaminase crosslinking and is insensitive to the known splice variation involving exon 10. Northern blot analysis of total hepatic RNA showed that the mutation did not effect expression of any of the three fibrinogen polypeptides (alpha, beta, and gamma). Levels of circulating the fibrinogen complex were determined to be unaffected in mutant mice by Western blot analysis and quantitative capture ELISA of plasma. (J:36873)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgg Mutation: 30 strains or lines available

References

• Original: J:36873 Holmback K, et al., Impaired platelet aggregation and sustained bleeding in mice lacking the fibrinogen motif bound by integrin alpha IIb beta 3. EMBO J. 1996 Nov 1;15(21):5760-71
• All: 9 reference(s)