Cntn1^tm1Brns
Targeted Allele Detail

Summary

• Symbol: Cntn1^tm1Brns
• Name: contactin 1; targeted mutation 1, Barbara Ranscht
• MGI ID: MGI:2384082
• Synonyms: CNTN^-
• Gene: Cntn1 Location: Chr15:91949034-92239834 bp, + strand Genetic Position: Chr15, 46.39 cM, cytoband F
• Alliance: Cntn1^tm1Brns page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58556
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by insertion of a PGK-neo cassette into exon 3 via homologous recombination. Absence of protein product in homozygous mutant animals was verified by Western blot analysis of brain homogenates and immunohistochemical analysis of cerebellum sections. Additionally, cerebellar granule cells cultured from homozygous mutants did not immunoprecipitate or stain for protein product. (J:58556)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cntn1 Mutation: 58 strains or lines available

References

• Original: J:58556 Berglund EO, et al., Ataxia and abnormal cerebellar microorganization in mice with ablated contactin gene expression. Neuron. 1999 Nov;24(3):739-50
• All: 13 reference(s)