Cd3e^tm1.1Lov
Targeted Allele Detail

Summary

• Symbol: Cd3e^tm1.1Lov
• Name: CD3 antigen, epsilon polypeptide; targeted mutation 1.1, Paul E Love
• MGI ID: MGI:2384123
• Synonyms: Cd3e-, Cd3e^delta, Cd3epsilon-
• Gene: Cd3e Location: Chr9:44910038-44920925 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Cd3e^tm1.1Lov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72144
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated by breeding mice homozygous for Cd3e^tm1Lov with transgenic mice expressing cre recombinase via the EIIa promoter, resulting in the excision of the floxed neo. Northern blot analysis of homozygous mutant mice showed normal levels of Cd3d and Cd3g transcript as well as the presence of a truncated Cd3e transcript. Sequencing of Cd3e cDNA derived from RT-PCR analysis showed the splicing of exons 4 to 7 (lacking the extracellular and transmembrane domains). Western blot analysis showed the absence of truncated protein product. (J:72144)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd3e Mutation: 54 strains or lines available

References

• Original: J:72144 DeJarnette JB, et al., Specific requirement for CD3epsilon in T cell development. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14909-14
• All: 13 reference(s)