Plp1^tm2Wst
Targeted Allele Detail

Summary

• Symbol: Plp1^tm2Wst
• Name: proteolipid protein (myelin) 1; targeted mutation 2, Wilhelm Stoffel
• MGI ID: MGI:2384127
• Synonyms: dm20/neo
• Gene: Plp1 Location: ChrX:135723420-135740482 bp, + strand Genetic Position: ChrX, 59.1 cM, cytoband F1-F2
• Alliance: Plp1^tm2Wst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21976
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: 105 bp of exon 3 were deleted to mimic the DM20 isoform and a neomycin resistance cassette was inserted into intron 3 to disrupt splicing of the gene product. Northern blot analysis confirmed the absence of normal transcripts in the brain of homozygous mutant animals. Western blot analysis and silver stained SDS-PAGE gels of myelin samples from homozygous mutants did not detect protein product of either isoform, PLP or DM20. (J:21976)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plp1 Mutation: 18 strains or lines available

References

• Original: J:21976 Boison D, et al., Disruption of the compacted myelin sheath of axons of the central nervous system in proteolipid protein-deficient mice. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11709-13
• All: 2 reference(s)