Mpz^tm1Msch
Targeted Allele Detail

Summary

• Symbol: Mpz^tm1Msch
• Name: myelin protein zero; targeted mutation 1, Melitta Schachner
• MGI ID: MGI:2384133
• Synonyms: P0-
• Gene: Mpz Location: Chr1:170978282-170988699 bp, + strand Genetic Position: Chr1, 79.05 cM
• Alliance: Mpz^tm1Msch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:3234
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 encoding the signal peptide was replaced with a neomycin resistance gene via homologous recombination. Southern blot analysis revealed several copies of the targeting vector had integrated as a concatemer in a head-to-tail manner. Western blot analysis of peripheral nerve proteins from homozygous mutant animals confirmed the absence of gene expression. The null allele was further verified by immunohistochemical analysis of femoral nerve sections from homozygous mutant animals using a polyclonal antibody to the protein. No immunoreactive staining was observed. (J:3234)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mpz Mutation: 28 strains or lines available

References

• Original: J:3234 Giese KP, et al., Mouse P0 gene disruption leads to hypomyelination, abnormal expression of recognition molecules, and degeneration of myelin and axons. Cell. 1992 Nov 13;71(4):565-76
• All: 49 reference(s)