Tg(EEF1A1-SHC1*)1Ravi
Transgene Detail

Summary

• Symbol: Tg(EEF1A1-SHC1*)1Ravi
• Name: transgene insertion 1, Kodi S Ravichandran
• MGI ID: MGI:2384188
• Synonyms: ShcFFF, STOPfloxShcFFF
• Transgene: Tg(EEF1A1-SHC1*)1Ravi Location: unknown
• Alliance: Tg(EEF1A1-SHC1*)1Ravi page

Transgene origin

• Strain of Origin: 129

Transgene description

• Transgene Type: Transgenic (Conditional ready, Dominant negative, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(EEF1A1-SHC1*)1Ravi expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SHC1 (6464)

  Shc1	(MGI:98296)

• Mutation details: A construct was generated in which a human EF-1alpha promoter was separated from a modified human SHC1 cDNA fused to an IRES-GFP by a floxed stop codon. The floxed stop codon allowed transcriptional control via the expression of cre recombinase. SHC1 codons encoding three tyrosine residues (positions 239, 240, 317) that are phosphorylated after cross linking were modified to encode phenylalanine. This creates a dominant-negative protein in certain signaling pathways. Breeding of mice carrying this allele with transgenic mice expressing cre resulted in the expression of the modified SHC1, though not GFP. (J:78180)
• Inheritance: Dominant

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:78180 Zhang L, et al., A nonredundant role for the adapter protein Shc in thymic T cell development. Nat Immunol. 2002 Aug;3(8):749-55
• All: 7 reference(s)