Irak3^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Irak3^tm1Flv
• Name: interleukin-1 receptor-associated kinase 3; targeted mutation 1, Richard A Flavell
• MGI ID: MGI:2384197
• Synonyms: IRAK-M^-
• Gene: Irak3 Location: Chr10:119977553-120038035 bp, - strand Genetic Position: Chr10, 67.94 cM, cytoband D2
• Alliance: Irak3^tm1Flv page

Small size and reduced total bone mineral density in Irak3^tm1Flv/Irak3^tm1Flv mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78189
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 1.2 kb of sequence were replaced with a floxed neo cassette. The deleted region contained 3 exons encoding two thirds of the kinase domain. While Western blot analysis of LPS-stimulated bone marrow macrophage extracts using a carboxy terminal antibody confirmed the absence of normal protein in homozygous mutant mice, truncated transcript was detected by Northern blot analysis using an amino terminal probe. Sequence analysis of RT-PCR products revealed an in frame stop codon generated by aberrant splicing of the endogenous gene and the neo transgene. (J:78189)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Irak3 Mutation: 103 strains or lines available

References

• Original: J:78189 Kobayashi K, et al., IRAK-M is a negative regulator of Toll-like receptor signaling. Cell. 2002 Jul 26;110(2):191-202
• All: 30 reference(s)