Pparg^tm1Gonz
Targeted Allele Detail

Summary

• Symbol: Pparg^tm1Gonz
• Name: peroxisome proliferator activated receptor gamma; targeted mutation 1, Frank J Gonzales
• MGI ID: MGI:2384211
• Synonyms: t
• Gene: Pparg Location: Chr6:115337912-115467360 bp, + strand Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
• Alliance: Pparg^tm1Gonz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75777
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was introduced 1 kb upstream of exon 2 and a floxed PGK-neo cassette was introduced 0.75 kb downstream of exon 2 via homologous recombination. Exon 2 encodes the DNA-binding domain of the gene. (J:75777)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pparg Mutation: 41 strains or lines available

Notes

Homozygous mutant animals could not be recovered. The insertion of the PGK-neo cassette into an intronic region appears to disrupt the gene and results in homozygous embryonic lethality.

References

• Original: J:75777 Akiyama TE, et al., Conditional disruption of the peroxisome proliferator-activated receptor gamma gene in mice results in lowered expression of ABCA1, ABCG1, and apoE in macrophages and reduced cholesterol efflux. Mol Cell Biol. 2002 Apr;22(8):2607-19
• All: 9 reference(s)