Ntrk2^tm1Lfr
Targeted Allele Detail

Summary

• Symbol: Ntrk2^tm1Lfr
• Name: neurotrophic tyrosine kinase, receptor, type 2; targeted mutation 1, Louis F Reichardt
• MGI ID: MGI:2384391
• Synonyms: fBZ, trkB^fbz, TrkB^lox
• Gene: Ntrk2 Location: Chr13:58954383-59281784 bp, + strand Genetic Position: Chr13, 31.2 cM
• Alliance: Ntrk2^tm1Lfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:69123
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The first exon (exon S) was replaced with a floxed full-length cDNA copy of the gene followed by a PGK-neo-loxP cassette and a tau-lacZ cassette. The PGK-neo-loxP cassette was removed in correctly targeted ES cells via transient expression of cre recombinase, leaving the floxed cDNA and tau-lacZ cassette intact. Expression of the floxed cDNA is under the control of the endogenous promoter. The tau-lacZ sequence is not transcribed due to SV40 polyadenylation signals and multiple stop codons following the floxed cDNA. Northern blot analysis of brain from homozygous mutant animals shows the presence of only full-length transcripts. Multiple alternative transcripts observed in wild-type animals are absent. Gene expression and protein expression in mutant animals is approximately 33% and 24% that of wild-type, respectively. Immunohistochemical analysis demonstrated that only full-length protein, and no protein isoforms, are expressed in mutant animals, and the pattern of expression is similar to wild-type. (J:69123)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ntrk2 Mutation: 66 strains or lines available

References

• Original: J:69123 Xu B, et al., The role of brain-derived neurotrophic factor receptors in the mature hippocampus: modulation of long-term potentiation through a presynaptic mechanism involving TrkB. J Neurosci. 2000 Sep 15;20(18):6888-97
• All: 20 reference(s)