Gast^tm1Psi
Targeted Allele Detail

Summary

• Symbol: Gast^tm1Psi
• Name: gastrin; targeted mutation 1, Pomila Singh
• MGI ID: MGI:2384400
• Synonyms: GAS-KO
• Gene: Gast Location: Chr11:100225233-100227822 bp, + strand Genetic Position: Chr11, 63.46 cM
• Alliance: Gast^tm1Psi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77985
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion of a PGK-neo cassette into exon 2 via homologous recombination. Loss of gene expression was observed in homozygous mutant animals by RT-PCR analysis of antral mucosa samples using primers to exons 1 and 3. Protein product was undetectable in plasma from homozygous mutants. (J:77985)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gast Mutation: 18 strains or lines available

References

• Original: J:77985 Cobb S, et al., Deletion of functional gastrin gene markedly increases colon carcinogenesis in response to azoxymethane in mice. Gastroenterology. 2002 Aug;123(2):516-30
• All: 2 reference(s)