Slc8a1^tm1Hssh
Targeted Allele Detail

Summary

• Symbol: Slc8a1^tm1Hssh
• Name: solute carrier family 8 (sodium/calcium exchanger), member 1; targeted mutation 1, Hee-Sup Shin
• MGI ID: MGI:2384413
• Synonyms: NCX1^-
• Gene: Slc8a1 Location: Chr17:81680534-82045806 bp, - strand Genetic Position: Chr17, 51.29 cM
• Alliance: Slc8a1^tm1Hssh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74096
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK-neo-poly(A) cassette was inserted at the NcoI site located in the first coding exon. This insertion disrupted the coding sequence after amino acid 247, predicting to result in premature termination of the encoded protein. RT-PCR analysis on RNA derived from E9.5 homozygous embryos demonstrated that no detectable transcript was expressed from this allele. (J:74096)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc8a1 Mutation: 55 strains or lines available

References

• Original: J:74096 Cho CH, et al., The Na+ -Ca2+ exchanger is essential for embryonic heart development in mice. Mol Cells. 2000 Dec 31;10(6):712-22
• All: 2 reference(s)