Pitx2^tm1Rsd
Targeted Allele Detail

Summary

• Symbol: Pitx2^tm1Rsd
• Name: paired-like homeodomain transcription factor 2; targeted mutation 1, Michael G Rosenfeld
• MGI ID: MGI:2384494
• Synonyms: Pitx2^-, Pitx2^LacZ
• Gene: Pitx2 Location: Chr3:128993527-129013240 bp, + strand Genetic Position: Chr3, 57.84 cM
• Alliance: Pitx2^tm1Rsd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57673
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of the entire homeodomain and C-terminus with an in-frame lacZ-neo cassette via homologous recombination. Southern blot analysis identified homozygous mutant embryos up to E15. X-gal staining of mutant embryos demonstrated the expression pattern of the lacZ targeted insert is similar to that of endogenous protein. (J:57673)

Disease models

Expression

In Mice Carrying this Mutation:	342 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pitx2 Mutation: 39 strains or lines available

References

• Original: J:57673 Lin CR, et al., Pitx2 regulates lung asymmetry, cardiac positioning and pituitary and tooth morphogenesis. Nature. 1999 Sep 16;401(6750):279-82
• All: 10 reference(s)