Kcna5^{tm1(Kcna1)Lndn}
Targeted Allele Detail

Summary

• Symbol: Kcna5^{tm1(Kcna1)Lndn}
• Name: potassium voltage-gated channel, shaker-related subfamily, member 5; targeted mutation 1, Barry London
• MGI ID: MGI:2384510
• Synonyms: SWAP
• Gene: Kcna5 Location: Chr6:126509514-126512375 bp, - strand Genetic Position: Chr6, 61.35 cM
• Alliance: Kcna5^{tm1(Kcna1)Lndn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78122
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing rat Kcna1 sequence appended with a hemagglutinin tag (HA) and neo was incorporated at the enodogenous locus via homologous recombination. The rat sequence was inserted such that it would replace the Kcna5 coding sequence and be expressed by the Kcna5 promoter. Northern blot analysis confirmed the absence of Kcna5, while the presence of the rat Kcna1 was detected by RT-PCR analysis. HA epitope directed antibodies showed a 62 kDa protein in the heart tissue obtained from mutant mice, but not in brain tissue. (J:78122)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcna5 Mutation: 34 strains or lines available

References

• Original: J:78122 London B, et al., Targeted replacement of KV1.5 in the mouse leads to loss of the 4-aminopyridine-sensitive component of I(K,slow) and resistance to drug-induced qt prolongation. Circ Res. 2001 May 11;88(9):940-6
• All: 2 reference(s)