Gfap^tm4Ito
Targeted Allele Detail

Summary

• Symbol: Gfap^tm4Ito
• Name: glial fibrillary acidic protein; targeted mutation 4, Shigeyoshi Itohara
• MGI ID: MGI:2384721
• Synonyms: Gfap^hm3
• Gene: Gfap Location: Chr11:102778162-102791368 bp, - strand Genetic Position: Chr11, 66.48 cM, cytoband D
• Alliance: Gfap^tm4Ito page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78341
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: The 5' portion of exon 1 was replaced with corresponding human sequence mutagenized to contain substitution mutations at all 5 phosphorylation sites, Thr7, Ser8, Ser13, Ser17, and Ser38 (human positions). The incorporation of the specific mutations was verified by sequence analysis as well as by Western blot analysis using antibodies directed toward the phosphorylation sites. Northern blot analysis of total brain RNA showed that transcript levels were unaffected in mutant mice. Immunohistochemical analysis of brain tissue showed that while the distribution of protein was conserved in mutant mice, protein levels were reduced in various regions. (J:78341)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gfap Mutation: 48 strains or lines available

References

• Original: J:78341 Takemura M, et al., Protective role of phosphorylation in turnover of glial fibrillary acidic protein in mice. J Neurosci. 2002 Aug 15;22(16):6972-9
• All: 1 reference(s)