Hif1a^tm1Rsjo
Targeted Allele Detail

Summary

• Symbol: Hif1a^tm1Rsjo
• Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 1, Randall S Johnson
• MGI ID: MGI:2385358
• Synonyms: Hif1alpha^-, HIF-1alpha^-
• Gene: Hif1a Location: Chr12:73948186-73994304 bp, + strand Genetic Position: Chr12, 31.99 cM
• Alliance: Hif1a^tm1Rsjo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48084
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence in exon 2 encoding the basic helix-loop-helix domain was replaced with a neomycin selection cassette. The absence of transcript and functional protein was shown by Northern blot analysis and an electromobility shift assay. (J:48084)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hif1a Mutation: 50 strains or lines available

References

• Original: J:48084 Ryan HE, et al., HIF-1 alpha is required for solid tumor formation and embryonic vascularization. EMBO J. 1998 Jun 1;17(11):3005-15
• All: 13 reference(s)