Hif1a^tm2Rsjo
Targeted Allele Detail

Summary

• Symbol: Hif1a^tm2Rsjo
• Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 2, Randall S Johnson
• MGI ID: MGI:2385361
• Gene: Hif1a Location: Chr12:73948186-73994304 bp, + strand Genetic Position: Chr12, 31.99 cM
• Alliance: Hif1a^tm2Rsjo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48084
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Sequence encoding the basic helix-loop-helix domain was disrupted by the insertion of a construct containing lacZ followed by a floxed neo cassette via homologous recombination. The neo cassette was excised by the transient transfection of targeted ES cells by cre recombinase. Expression of lacZ was visualized via Xgal staining. (J:48084)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hif1a Mutation: 50 strains or lines available

References

• Original: J:48084 Ryan HE, et al., HIF-1 alpha is required for solid tumor formation and embryonic vascularization. EMBO J. 1998 Jun 1;17(11):3005-15
• All: 6 reference(s)