Tg(Camk2a-Grin2b)1Jzt
Transgene Detail

Summary

• Symbol: Tg(Camk2a-Grin2b)1Jzt
• Name: transgene insertion 1, Joe Z Tsien
• MGI ID: MGI:2385435
• Synonyms: NR2B Tg, Tg-GluN2B
• Transgene: Tg(Camk2a-Grin2b)1Jzt Location: unknown
• Alliance: Tg(Camk2a-Grin2b)1Jzt page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Camk2a-Grin2b)1Jzt expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Grin2b (MGI:95821)

• Mutation details: The transgene consists of the full Grin2b gene driven by 8.5 kb of Camk2a promoter, active in the mouse forebrain. Northern blot analysis demonstrated enrichment of gene expression in the cortex and hippocampus of transgenic animals and Western blot analysis demonstrated 2X protein over-expression compared to wild-type animals. Furthermore, in situ hybridization showed enrichment of the transgene in the cortex, striatum, hippocampus, and amydala. A slight increase in expression of Grin1 protein was also detected. (J:57250)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two founder transgenic lines Tg-1 and Tg-2 were created and used in the study. Both founders exhibit similar expression patterns and levels of the transgene, and also display similar electrophysiological and behavioral phenotypes.

References

• Original: J:57250 Tang YP, et al., Genetic enhancement of learning and memory in mice [see comments]. Nature. 1999 Sep 2;401(6748):63-9
• All: 14 reference(s)