Enpp1^tm1Gdg
Targeted Allele Detail

Summary

• Symbol: Enpp1^tm1Gdg
• Name: ectonucleotide pyrophosphatase/phosphodiesterase 1; targeted mutation 1, J W Goding
• MGI ID: MGI:2385534
• Synonyms: Enpp1^-, PC-1-
• Gene: Enpp1 Location: Chr10:24513812-24588057 bp, - strand Genetic Position: Chr10, 12.26 cM
• Alliance: Enpp1^tm1Gdg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78590
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 9 was disrupted by the insertion of a neomycin selection cassette. RT-PCR analysis of total renal RNA showed the presence of an elongated transcript in homozygous mice, putatively resulting from a cryptic splice site within the neo transgene. Immunohistochemical analysis of epididymal sections showed an absence of encoded protein in homozygous mutant mice. (J:78590)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Enpp1 Mutation: 11 strains or lines available

References

• Original: J:78590 Sali A, et al., Germline deletion of the nucleoside triphosphate pyrophosphohydrolase (NTPPPH) plasma cell membrane glycoprotein (PC-1) produces abnormal calcification of periarticular tissues, in Proceedings of the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases. 1999:267-282.
• All: 19 reference(s)