Pla2g4a^tm1Tksh
Targeted Allele Detail

Summary

• Symbol: Pla2g4a^tm1Tksh
• Name: phospholipase A2, group IVA (cytosolic, calcium-dependent); targeted mutation 1, Takao Shimizu
• MGI ID: MGI:2385582
• Synonyms: cpla2-, cPLA2alpha, cPLA2 GIVA, IVA PLA2^-
• Gene: Pla2g4a Location: Chr1:149705369-149837041 bp, - strand Genetic Position: Chr1, 63.51 cM
• Alliance: Pla2g4a^tm1Tksh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39254
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of part of the exon encoding the lipase consensus motif and serine 228, a residue required for enzymatic activity. Absence of gene expression in the spleen of homozygous animals was verified by Northern blot and Western blot analyses. (J:39254)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pla2g4a Mutation: 62 strains or lines available

References

• Original: J:39254 Uozumi N, et al., Role of cytosolic phospholipase A2 in allergic response and parturition. Nature. 1997 Dec 11;390(6660):618-22
• All: 39 reference(s)