Mapt^tm1Hnd
Targeted Allele Detail

Summary

• Symbol: Mapt^tm1Hnd
• Name: microtubule-associated protein tau; targeted mutation 1, Hana N Dawson
• MGI ID: MGI:2385630
• Synonyms: MaptKO(Duke), TAU^-
• Gene: Mapt Location: Chr11:104122216-104222916 bp, + strand Genetic Position: Chr11, 67.79 cM, cytoband E1
• Alliance: Mapt^tm1Hnd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78649
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, encoding the translational start site, was replaced by a neomycin selection cassette via homologous recombination. RT-PCR analysis of total brain RNA obtained from homozygous mutant mice showed a lack of transcript produced by the targeted locus. The absence of encoded protein was verified by Western blot analysis of brain homogenates as well as by immunocytochemical analysis of coronal sections. (J:78649)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available
• Carrying any Mapt Mutation: 430 strains or lines available

References

• Original: J:78649 Dawson HN, et al., Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. J Cell Sci. 2001 Mar;114(Pt 6):1179-87
• All: 110 reference(s)