Rb1^tm2Tyj
Targeted Allele Detail

Summary

• Symbol: Rb1^tm2Tyj
• Name: RB transcriptional corepressor 1; targeted mutation 2, Tyler Jacks
• MGI ID: MGI:2385637
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm2Tyj page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:59268
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Insertion of a hygromycin resistance cassette into one allele of the Rb1 gene replaced part of intron 3 and introduced three nucleotide changes into exon 3, creating two termination codons and a new PstI site. This was accomplished in ES cells in which Rb1 had already been targeted. The resulting ES cells are Rb1^tm1Tyj/Rb1^tm2Tyj. (J:59268)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Rb1^tm2Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 111 strains or lines available

Notes

Homozygous mutants at this locus are embryonic lethal. ES cells homozygous for functional knockout of this locus were created so that phenotypes resulting from homozygous cells could be studied in adult chimeras. The contribution of double knockout cells to the composition of chimeric mice varied from 30 to 60%.

References

• Original: J:59268 Williams BO, et al., Extensive contribution of Rb-deficient cells to adult chimeric mice with limited histopathological consequences. EMBO J. 1994 Sep 15;13(18):4251-9
• All: 1 reference(s)