Hoxa2^{tm1(tetO)Mllo}
Targeted Allele Detail

Summary

• Symbol: Hoxa2^{tm1(tetO)Mllo}
• Name: homeobox A2; targeted mutation 1, Moises Mallo
• MGI ID: MGI:2385709
• Synonyms: Hoxa2^tetO
• Gene: Hoxa2 Location: Chr6:52139397-52141811 bp, - strand Genetic Position: Chr6, 25.4 cM
• Alliance: Hoxa2^{tm1(tetO)Mllo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71951
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: Seven copies of the tetracycline operator were inserted in the 5' untranslated region of the endogenous locus via homologous recombination. An RNAse protection assay revealed the inserted sequence to be approximately 100 bp upstream of the transcriptional start site. While quantitative analysis of the amount of transcript produced by the mutant allele showed a 55% reduction relative to the wild-type allele, in situ hybridization indicated that the pattern of expression was unaffected. (J:71951)

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxa2 Mutation: 21 strains or lines available

References

• Original: J:71951 Ohnemus S, et al., Different levels of Hoxa2 are required for particular developmental processes. Mech Dev. 2001 Oct;108(1-2):135-47
• All: 3 reference(s)