Lhx1^tm3.1Bhr
Targeted Allele Detail

Summary

• Symbol: Lhx1^tm3.1Bhr
• Name: LIM homeobox protein 1; targeted mutation 3.1, Richard R Behringer
• MGI ID: MGI:2385728
• Synonyms: lhx1^Bhrm2, lim1^m
• Gene: Lhx1 Location: Chr11:84409110-84416361 bp, - strand Genetic Position: Chr11, 51.31 cM
• Alliance: Lhx1^tm3.1Bhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63192
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted
• Mutation: Disruption caused by insertion of vector
• Mutation details: The neo cassette inserted into intron 1 of Lhx^tm3Bhr was excised by breeding with CMV-cre transgenic mice. The four missense mutations affecting conserved cysteine residues in exons 1 and 2, encoding the two LIM domains, were left intact. The conservation of the endogenous expression pattern was verified by whole mount in situ hybridization of homozygous mutant embryos. RT-PCR and sequence analyses showed normal expression and the exclusive presence of the missense mutations. Finally, immunocytochemical analysis confirmed the presence of protein in mutant mice. (J:63192)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lhx1 Mutation: 22 strains or lines available

References

• Original: J:63192 Cheah SS, et al., Requirement of LIM domains for LIM1 function in mouse head development. Genesis. 2000 May;27(1):12-21
• All: 1 reference(s)