Itga2b^tm1Tlr
Targeted Allele Detail

Summary

• Symbol: Itga2b^tm1Tlr
• Name: integrin alpha 2b; targeted mutation 1, Diana Tronik-Le Roux
• MGI ID: MGI:2385793
• Synonyms: alphaIIb^-
• Gene: Itga2b Location: Chr11:102344134-102360570 bp, - strand Genetic Position: Chr11, 66.29 cM
• Alliance: Itga2b^tm1Tlr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63961
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by insertion of a TK-PGK-neo cassette into exon 1 via homologous recombination. Northern blot analysis showed the absence of Itga2b expression and presence of TK transcripts in the bone marrow of homozygous mutant animals. Immunoblot analysis using an antibody directed against human Itga2b confirmed absence of protein product in platelet extracts from homozygous mutant mice. (J:63961)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itga2b Mutation: 45 strains or lines available

References

• Original: J:63961 Tronik-Le Roux D, et al., Thrombasthenic mice generated by replacement of the integrin alpha(IIb) gene: demonstration that transcriptional activation of this megakaryocytic locus precedes lineage commitment. Blood. 2000 Aug 15;96(4):1399-408
• All: 3 reference(s)