Per3^tm1Drw
Targeted Allele Detail

Summary

• Symbol: Per3^tm1Drw
• Name: period circadian clock 3; targeted mutation 1, David R Weaver
• MGI ID: MGI:2385797
• Synonyms: mPER3-deficient
• Gene: Per3 Location: Chr4:151088109-151129122 bp, - strand Genetic Position: Chr4, 81.53 cM
• Alliance: Per3^tm1Drw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63933
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 3 and part of exon 4 (encoding amino acids 92-154) with a PGK-neo cassette via homologous recombination. Introduction of PGK-neo introduces stop codons into all 3 reading frames. Western blot analysis of brain lysates from homozygous mutant animals using antibodies directed against the C-terminus of the protein confirmed the absence of protein expression. Immunoprecipitation assays also confirmed the absence of protein product. Northern blot and RT-PCR analysis detected mutant transcripts containing neo and lacking exon 3 and part of exon 4 in brain and skeletal muscles from homozygous mutants. (J:63933)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Per3 Mutation: 47 strains or lines available

References

• Original: J:63933 Shearman LP, et al., Targeted disruption of the mPer3 gene: subtle effects on circadian clock function. Mol Cell Biol. 2000 Sep;20(17):6269-75
• All: 28 reference(s)