Fosl1^tm1Wag
Targeted Allele Detail

Summary

• Symbol: Fosl1^tm1Wag
• Name: fos-like antigen 1; targeted mutation 1, Erwin F Wagner
• MGI ID: MGI:2385824
• Synonyms: Fra1^-
• Gene: Fosl1 Location: Chr19:5497726-5505966 bp, + strand Genetic Position: Chr19, 4.33 cM
• Alliance: Fosl1^tm1Wag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65292
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 3, all of intron 3, and part of exon 4 were replaced with a lacZ-neo derived cassette via homologous recombination, fusing lacZ in-frame with exon 3. The gene targeting event deletes most of the DNA-binding domain, the leucine zipper dimerization domain, and the last splice acceptor site. Homozygous mutant embryos were detected up to E11.5 by Southern blot analysis. (J:65292)

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fosl1 Mutation: 16 strains or lines available

Notes

ES cell line = D3 (129S2/SvPas) or R1 ((129X1/SvJ x 129S1/Sv)F1-Kitl⁺). Mutant animals derived from each cell line were analysed. Survival of homozygous embryos rescued when ES cells are injected into wild-type tetraploid blastocysts. - - J:65292

References

• Original: J:65292 Schreiber M, et al., Placental vascularisation requires the AP-1 component fra1. Development. 2000 Nov;127(22):4937-48
• All: 2 reference(s)