Vpreb1b^tm1Ilm
Targeted Allele Detail

Summary

• Symbol: Vpreb1b^tm1Ilm
• Name: V-set pre-B cell surrogate light chain 1B; targeted mutation 1, Inga-Lill Martensson
• MGI ID: MGI:2385833
• Synonyms: VpreB2^-
• Gene: Vpreb1b Location: Chr16:17798292-17799137 bp, + strand Genetic Position: Chr16, 11.14 cM
• Alliance: Vpreb1b^tm1Ilm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67609
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single copy of a construct in which the entire coding region was replaced with a hygromycin cassette was incorporated at the endogenous locus via homologous recombination. Transcript was undetected by RT-PCR analysis of bone marrow cells obtained from homozygous mutant mice. The absence of protein surface expression was confirmed by FACS analysis. (J:67609)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vpreb1b Mutation: 8 strains or lines available

Notes

ES cells heterozygous for Vpreb1^tm1Ilm were electroporated with the targeting vector. Two targeted mutant lines identified as ES94 and as ES163 were interbred to create this mutant lineage. This allele always exists in association with Vpreb1^tm1Ilm.

References

• Original: J:67609 Mundt C, et al., Loss of precursor B cell expansion but not allelic exclusion in VpreB1/VpreB2 double-deficient mice. J Exp Med. 2001 Feb 19;193(4):435-45
• All: 3 reference(s)