Myl2^tm1(cre)Krc
Targeted Allele Detail

Summary

• Symbol: Myl2^tm1(cre)Krc
• Name: myosin, light polypeptide 2, regulatory, cardiac, slow; targeted mutation 1, Kenneth R Chien
• MGI ID: MGI:2385922
• Synonyms: MLC-2v -, Mlc2v-cre, MLC2v-cre, MLC2vcreKI
• Gene: Myl2 Location: Chr5:122239014-122251535 bp, + strand Genetic Position: Chr5, Syntenic
• Alliance: Myl2^tm1(cre)Krc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45125
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Myl2^tm1(cre)Krc expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myl2 (MGI:97272)

• Mutation details: Part of exon 1 and all of exon 2 were replaced with a cre gene and a floxed PGK-neo-tk cassette via homologous recombination. Western blot analysis showed that homozygous embryos did not express Myl2 protein product. The knocked in cre gene is expressed by the Myl2 promoter in ventricular tissue starting at day E7.5. (J:45125)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myl2 (mouse) Summary of all recombinase alleles driven by Myl2. Comparative matrix view of recombinase activities Alliance mouse Myl2 gene page

Disease models

Expression

In Mice Carrying this Mutation:

25 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Myl2 Mutation: 22 strains or lines available

References

• Original: J:45125 Chen J, et al., Selective requirement of myosin light chain 2v in embryonic heart function. J Biol Chem. 1998 Jan 9;273(2):1252-6
• All: 137 reference(s)