Rxrg^tm1Ipc
Targeted Allele Detail

Summary

• Symbol: Rxrg^tm1Ipc
• Name: retinoid X receptor gamma; targeted mutation 1, Pierre Chambon
• MGI ID: MGI:2385928
• Synonyms: Rxrgamma^-, Xgamma
• Gene: Rxrg Location: Chr1:167425953-167467192 bp, + strand Genetic Position: Chr1, 74.99 cM
• Alliance: Rxrg^tm1Ipc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:34890
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced exons 3 and 4, which encode the DNA binding domain and are common to both isoforms generated from this locus. While normal transcript was undetected several types of aberrant transcript were detected in homozygous mutant mice by Northern blot and RT-PCR analyses, including 1.6 and 3.0 kb fragments derived from the exon 1b isoform and several others derived from the exon 1a isoform. The 1.6 kb fragment involves an aberrant splice between exon 1b and exon 5, resulting in an isolated ligand binding coding region. The 3.0 kb fragment is believed to be the result of cryptic splicing within the neo transgene or an intron. All of the aberrant transcripts lacked the DNA binding domain. (J:34890)

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rxrg Mutation: 29 strains or lines available

References

• Original: J:34890 Krezel W, et al., RXR gamma null mice are apparently normal and compound RXR alpha +/-/RXR beta -/-/RXR gamma -/- mutant mice are viable. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9010-4
• All: 16 reference(s)