Apoe^{tm1(APOE_i4)Sfu}
Targeted Allele Detail

Summary

• Symbol: Apoe^{tm1(APOE_i4)Sfu}
• Name: apolipoprotein E; targeted mutation 1, Shinobu C Fujita
• MGI ID: MGI:2385931
• Synonyms: 4/4, 4/4 KI, ApoE4-KI, Apoe^tm1(APOE)Sfu, Apoe^{tm3(APOE4)sfu}, epsilon4 KI
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^{tm1(APOE_i4)Sfu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60590
• Parent Cell Line: E14TG2aIV (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Apoe^{tm1(APOE_i4)Sfu} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

• Mutation details: The human APOE isoform 4 cDNA and a floxed neo cassette were knocked into the mouse Apoe locus via homologous recombination. The knock in event deleted part of exon 2, all of exon 3, and part of exon 4 of the mouse Apoe sequence. Expression of the human gene was confirmed by cDNA sequence analysis of liver mRNA and Western blot analysis of serum proteins from mutant animals. Immunohistochemistry of brain sections showed that the human gene was expressed in astrocytes and neutropil of the hippocampus, and in the neocortex. (J:60590)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 156 strains or lines available

References

• Original: J:60590 Hamanaka H, et al., Altered cholesterol metabolism in human apolipoprotein E4 knock-in mice. Hum Mol Genet. 2000 Feb 12;9(3):353-61
• All: 15 reference(s)