Vamp3^tm1Jpes
Targeted Allele Detail

Summary

• Symbol: Vamp3^tm1Jpes
• Name: vesicle-associated membrane protein 3; targeted mutation 1, Jeffrey E Pessin
• MGI ID: MGI:2385941
• Synonyms: ceb^ko, cellubrevin/VAMP3^-, Vamp3^tm1Jepn
• Gene: Vamp3 Location: Chr4:151131762-151142410 bp, - strand Genetic Position: Chr4, 81.53 cM, cytoband E1
• Alliance: Vamp3^tm1Jpes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67364
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 7.8 kb of sequence were replaced with a neomycin selection cassette. The deleted region included exon 2, which contains the guanosine of the translational start codon, and exon 3, whcih encodes the entire coiled-coil domain. Western blot analysis of homozygous mutant mice showed an absence of the normal protein product. (J:67364)

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vamp3 Mutation: 17 strains or lines available

References

• Original: J:67364 Yang C, et al., VAMP3 null mice display normal constitutive, insulin- and exercise-regulated vesicle trafficking. Mol Cell Biol. 2001 Mar;21(5):1573-80
• All: 20 reference(s)