Nras^tm1Rak
Targeted Allele Detail

Summary

• Symbol: Nras^tm1Rak
• Name: neuroblastoma ras oncogene; targeted mutation 1, Raju Kucherlapati
• MGI ID: MGI:2385944
• Synonyms: N-ras-
• Gene: Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Nras^tm1Rak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:23318
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 2.2 kb of sequence from the 3' portion of exon 2 were replaced with a neomycin selection cassette via homologous recombination. The deleted sequence encoded two thirds of the carboxy terminal amino acids. Protein was undetected by Western blot analysis of extracts obtained from the lungs of homozygous mutant mice. (J:23318)

Expression

In Mice Carrying this Mutation:

15 assay results

Tumor Data

List all tumor models in MMHCdb carrying Nras^tm1Rak

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nras Mutation: 44 strains or lines available

References

• Original: J:23318 Umanoff H, et al., The murine N-ras gene is not essential for growth and development. Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1709-13
• All: 21 reference(s)