Has2^tm1Jam
Targeted Allele Detail

Summary

• Symbol: Has2^tm1Jam
• Name: hyaluronan synthase 2; targeted mutation 1, John A McDonald
• MGI ID: MGI:2386077
• Synonyms: Has2^-
• Gene: Has2 Location: Chr15:56529023-56557935 bp, - strand Genetic Position: Chr15, 23.31 cM
• Alliance: Has2^tm1Jam page

Growth retardation and yolk sac and somite abnormalities in Has2^tm1Jam/Has2^tm1Jam embryos

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63748
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of the end of intron 3 and the first 60 codons of the cytoplasmic catalytic domain containing exon 4 with a PGK-neo cassette via homologous recombination. Homozygous mutant embryos were observed at days E9.5-E11.5 and RT-PCR analysis of embryonic mRNA detected truncated mutant transcripts. (J:63748)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Has2 Mutation: 35 strains or lines available

References

• Original: J:63748 Camenisch TD, et al., Disruption of hyaluronan synthase-2 abrogates normal cardiac morphogenesis and hyaluronan-mediated transformation of epithelium to mesenchyme [see comments]. J Clin Invest. 2000 Aug;106(3):349-60
• All: 4 reference(s)