P2rx7^tm1Gab
Targeted Allele Detail

Summary

• Symbol: P2rx7^tm1Gab
• Name: purinergic receptor P2X, ligand-gated ion channel, 7; targeted mutation 1, Christopher A Gabel
• MGI ID: MGI:2386080
• Synonyms: P2X7-, P2X7 KO, P2X7R^-, P2X7R^Delta506-532, Pfizer P2X7^-
• Gene: P2rx7 Location: Chr5:122781974-122829495 bp, + strand Genetic Position: Chr5, 62.5 cM, cytoband F
• Alliance: P2rx7^tm1Gab page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66835
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding amino acids 506 through 532 was replaced by the insertion of a neomycin selection cassette. Transcript was undetected by Northern blot analysis of bone marrow mast cells isolated from homozygous mutant mice. Western blot analysis of homozygous mutant peritoneal macrophages showed an absence of normal and truncated protein. Subsequent analysis found low levels of expression of the 13b isoform in the brain, salivary gland and spleen as well as a C-terminal truncated variant transcript. (J:66835, J:194518)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any P2rx7 Mutation: 54 strains or lines available

References

• Original: J:66835 Solle M, et al., Altered cytokine production in mice lacking P2X(7) receptors. J Biol Chem. 2001 Jan 5;276(1):125-32
• All: 253 reference(s)