Nsmaf^tm1Mkr
Targeted Allele Detail

Summary

• Symbol: Nsmaf^tm1Mkr
• Name: neutral sphingomyelinase (N-SMase) activation associated factor; targeted mutation 1, Martin Kronke
• MGI ID: MGI:2386083
• Synonyms: FAN -, FAN-deficient
• Gene: Nsmaf Location: Chr4:6396207-6454271 bp, - strand Genetic Position: Chr4, 2.91 cM, cytoband A1
• Alliance: Nsmaf^tm1Mkr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54972
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of part of exon 2 with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of tail fibroblast and thymocyte mRNA. (J:54972)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nsmaf Mutation: 45 strains or lines available

References

• Original: J:54972 Kreder D, et al., Impaired neutral sphingomyelinase activation and cutaneous barrier repair in FAN-deficient mice. EMBO J. 1999 May 4;18(9):2472-9
• All: 4 reference(s)