Epor^tm1.3Jtp
Targeted Allele Detail

Summary

• Symbol: Epor^tm1.3Jtp
• Name: erythropoietin receptor; targeted mutation 1.3, Josef T Prchal
• MGI ID: MGI:2386096
• Synonyms: mthEPOR^{flox-neo(arrow)}, mthEPOR^neo
• Gene: Epor Location: Chr9:21870193-21874802 bp, - strand Genetic Position: Chr9, 7.93 cM
• Alliance: Epor^tm1.3Jtp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67205
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Epor^tm1.3Jtp expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EPOR (2057)

  Epor	(MGI:95408)

• Mutation details: This allele is a derivative of Epor^tm1Jtp in which an exchange targeting vector was used to replace the PGK-Hprt minigene. The exchange vector contains a mutated sequence of the human gene from the translational start site to the stop codon mutation, and a floxed PGK-neomycin resistance cassette inserted in reverse orientation into intron 6. The human sequence carrying a C5964G transversion mutation in exon 8 was subcloned from cDNA from polycythemia patients. (J:67205)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epor Mutation: 27 strains or lines available

Notes

Heterozygous mice (Epor⁺/Epor^tm1.3Jtp) of this genotype possess one copy of the wild-type mouse allele and one copy of the mutated human gene containing a floxed neomycin cassette.

Homozygous mice (Epor^tm1.3Jtp/Epor^tm1.3Jtp) of this genotype possess two copies of the mutated human gene containing a floxed neomycin cassette. Expresion analysis revealed that the gene was silenced, probably as a result of the retention of the neomycin cassette.

References

• Original: J:67205 Divoky V, et al., Mouse model of congenital polycythemia: Homologous replacement of murine gene by mutant human erythropoietin receptor gene. Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):986-91
• All: 1 reference(s)