Pou2af1^tm1Pjln
Targeted Allele Detail

Summary

• Symbol: Pou2af1^tm1Pjln
• Name: POU domain, class 2, associating factor 1; targeted mutation 1, Peter J Nielsen
• MGI ID: MGI:2386101
• Synonyms: BOB.1/OBF.1-
• Gene: Pou2af1 Location: Chr9:51125008-51151380 bp, + strand Genetic Position: Chr9, 27.95 cM
• Alliance: Pou2af1^tm1Pjln page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:37085
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 4, encompassing approximately 60% of the coding region, were replaced with a neomycin selection cassette. The deleted region encoded the amino terminal activation domain and a portion of the carboxy terminal domain. RT-PCR analysis of total spleen RNA showed an absence of transcript in homozygous mutant mice. (J:37085)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pou2af1 Mutation: 15 strains or lines available

References

• Original: J:37085 Nielsen PJ, et al., B lymphocytes are impaired in mice lacking the transcriptional co-activator Bob1/OCA-B/OBF1. Eur J Immunol. 1996 Dec;26(12):3214-8
• All: 9 reference(s)