Smarca4^tm1Mag
Targeted Allele Detail

Summary

• Symbol: Smarca4^tm1Mag
• Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; targeted mutation 1, Terry Magnuson
• MGI ID: MGI:2386105
• Synonyms: Brg1^-, Brg1^null
• Gene: Smarca4 Location: Chr9:21527465-21615526 bp, + strand Genetic Position: Chr9, 7.84 cM
• Alliance: Smarca4^tm1Mag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66861
• Parent Cell Line: CT129 (ES Cell)
• Strain of Origin: 129S/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced sequence encoding part of the DNA-dependent ATPase domain. The deleted region encoded helicase motif 1, which contains the ATP binding site, and helicase motif 1a. The allele was expected to encode a chimeric protein, though while Northern blot analysis identified transcripts containing both endogenous and neo transgene elements, protein was undetected by Western blot analysis. (J:66861)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Smarca4^tm1Mag

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Smarca4 Mutation: 110 strains or lines available

References

• Original: J:66861 Bultman S, et al., A Brg1 null mutation in the mouse reveals functional differences among mammalian SWI/SNF complexes. Mol Cell. 2000 Dec;6(6):1287-95
• All: 20 reference(s)