Rorc^tm1Amj
Targeted Allele Detail

Summary

• Symbol: Rorc^tm1Amj
• Name: RAR-related orphan receptor gamma; targeted mutation 1, Anton M Jetten
• MGI ID: MGI:2386114
• Synonyms: RORgamma^-
• Gene: Rorc Location: Chr3:94280106-94305583 bp, + strand Genetic Position: Chr3, 40.56 cM, cytoband F2
• Alliance: Rorc^tm1Amj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64381
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The DNA-binding domain, the hinge domain, and part of the ligand-binding domain (amino acids 25-309) encoded by exons 3-6 were replaced with a PGK-neo cassette via homologous recombination. Northern blot analysis of liver, thymus, and kidney tissue using a probe spanning exons 3-6 confirmed the absence of the targeted region in homozygous mutant animals. When analyzed using a full-length Rorc probe 2 species of mutant transcripts, probably due to alternative splicing events, were detected in homozygous mutants. (J:64381)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rorc Mutation: 44 strains or lines available

References

• Original: J:64381 Kurebayashi S, et al., Retinoid-related orphan receptor gamma (RORgamma) is essential for lymphoid organogenesis and controls apoptosis during thymopoiesis. Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10132-7
• All: 24 reference(s)