Col15a1^tm1Pih
Targeted Allele Detail

Summary

• Symbol: Col15a1^tm1Pih
• Name: collagen, type XV, alpha 1; targeted mutation 1, Taina Pihlajaniemi
• MGI ID: MGI:2386162
• Synonyms: Col15a1^-
• Gene: Col15a1 Location: Chr4:47208161-47313167 bp, + strand Genetic Position: Chr4, 26.01 cM, cytoband B1-B3
• Alliance: Col15a1^tm1Pih page

Col15a1^tm1Pih/Col15a1^tm1Pih muscles display areas of degeneration, regeneration and variation in fiber size

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67212
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was introduced ~900 bp upstream of exon 1 and a loxP-flanked neomycin resistance cassette 120 bp downstream of exon 2. Cre recombinase deleted exons 1 and 2, encoding the first 33 amino acids of the protein, and the selection cassette, leaving behind a single loxP site. This allele was derived from the same targeting event as Col15a1^tm2.1Pih. The absence of transcript and protein was verified by Northern blot and immunohistochemical analyses of homozygous mutant mice. (J:67212)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Col15a1^tm1Pih

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Col15a1 Mutation: 71 strains or lines available

References

• Original: J:67212 Eklund L, et al., Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice. Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1194-9
• All: 8 reference(s)